NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=10D;
DOI=10.1093/dnares/10.2.67; PubMed=12755171 [NCBI, ExPASy, EBI, Israel, Japan]
Ohta N., Matsuzaki M., Misumi O., Miyagishima S.-Y., Nozaki H., Tanaka K., Shin-i T., Kohara Y., Kuroiwa T.;
"Complete sequence and analysis of the plastid genome of the unicellular red alga Cyanidioschyzon merolae.";
DNA Res. 10:67-77(2003).

Comments

FUNCTION: Catalyzes the formation of the isocyclic ring in chlorophyll biosynthesis. Mediates the cyclase reaction, which results in the formation of divinylprotochlorophyllide (Pchlide) characteristic of all chlorophylls from magnesium-protoporphyrin IX 13-monomethyl ester (MgPMME) (By similarity).
CATALYTIC ACTIVITY: Magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O₂ = 13¹-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP⁺ + H₂O.
CATALYTIC ACTIVITY: 13¹-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O₂ = 13¹-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADP⁺ + 2 H₂O.
CATALYTIC ACTIVITY: 13¹-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O₂ = divinylprotochlorophyllide + NADP⁺ + 2 H₂O.
COFACTOR: Iron (By similarity).
PATHWAY: Porphyrin metabolism; chlorophyll biosynthesis (light-independent).
SUBCELLULAR LOCATION: Plastid, chloroplast.
SIMILARITY: Belongs to the acsF family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AB002583; BAC76217.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_849055.1; -.

3D structure databases

ModBase

Q85FX6.

Ontologies

GO:0005506;	Molecular function: iron ion binding (inferred from electronic annotation from HAMAP).
GO:0048529;	Molecular function: magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase activity (inferred from electronic annotation from HAMAP).
GO:0015979;	Biological process: photosynthesis (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_01840; -; 1.
PBIL [Tree]

InterPro

IPR008434; AcsF.
IPR003251; Rubrerythrin.
Graphical view of domain structure.

Pfam

PF02915; Rubrerythrin; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR02029; AcsF; 1.

BLOCKS

Q85FX6.

Genome annotation databases

GeneID

845087; -.

Other

ProtoNet

Q85FX6.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Chlorophyll biosynthesis; Chloroplast; Iron; Metal-binding; NADP; Oxidoreductase; Photosynthesis; Plastid.

Features

Feature table viewer

`Key`	`From To`	`Length`		`Description`	`FTId`
`CHAIN`	`1 335`	`335`		`Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase.`	`PRO_0000217542`

Sequence information

Length: 335 AA [This is the length of the unprocessed precursor]

Molecular weight: 40024 Da [This is the MW of the unprocessed precursor]

CRC64: BE3A1610AABF2915 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MQTAVQTQTT VQTLLTPRFY TTDFDQMAKY DISKNRAEIE AIVNEFRKDY NQTHFIRDDE 

        70         80         90        100        110        120 
FDQVWTQMPE SKRQWFKEFL ERSCTAEFSG FLLYKELSRR LKDTNPLLAE GFGYMSRDEA 

       130        140        150        160        170        180 
RHAGFLNKAM ADFGVSLDLG FLTKHRQYTF FAPKFIFYAT YLSEKIGYWR YITIYRHLEA 

       190        200        210        220        230        240 
HPKWSVYPIF KFFEKWCQDE NRHGDFFAAI LKAQPKWITG WASRLWCRFF LLSVFVTMYL 

       250        260        270        280        290        300 
NDLQRKDFYQ LIGLDARAFD WHVIRKTNES AARLFPVVLD VDHVDFVRLM DECACAYAHW 

       310        320        330 
RETQSVLSLA KIFACLFKLY VIKPINTQAL WNTIK

Q85FX6 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools