NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
Ferretti L., Uboldi C., Del Vecchio I., Eggen A., Brunner R., Iannuzzi L.;
"Comparative mapping of the bovine SPRN locus.";
Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.

[2]

NUCLEOTIDE SEQUENCE [MRNA] OF 62-512, PROTEIN SEQUENCE OF 7-27; 57-76 AND 497-511, AND CHARACTERIZATION.
TISSUE=Liver;
DOI=10.1074/jbc.M302149200; PubMed=12660232 [NCBI, ExPASy, EBI, Israel, Japan]
Wu T., Yankovskaya V., McIntire W.S.;
"Cloning, sequencing, and heterologous expression of the murine peroxisomal flavoprotein, N(1)-acetylated polyamine oxidase.";
J. Biol. Chem. 278:20514-20525(2003).

Comments

FUNCTION: Flavoenzyme which catalyzes the oxidation of N(1)-acetylspermine to spermidine and is thus involved in the polyamine back-conversion. Can also oxidize N(1)-acetylspermidine to putrescine. Substrate specificity: N(1)-acetylspermine = N(1)-acetylspermidine > N(1),N(12)-diacylspermine >> spermine. Does not oxidize spermidine. Plays an important role in the regulation of polyamine intracellular concentration.
CATALYTIC ACTIVITY: N¹-acetylspermine + O₂ + H₂O = N¹-acetylspermidine + 3-aminopropanal + H₂O₂.
COFACTOR: FAD (By similarity).
PATHWAY: Amine and polyamine metabolism; spermine metabolism .
SUBUNIT: Monomer (By similarity).
SUBCELLULAR LOCATION: Peroxisome (By similarity). Cytoplasm (By similarity).
MISCELLANEOUS: Oxidizes N(1)-acetylated polyamines on the exo-side of their N(4)-amino groups. Plant PAO oxidizes spermine on the endo-side of the N(4)-nitrogen (By similarity).
SIMILARITY: Belongs to the flavin monoamine oxidase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

DQ058607; AAY83881.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
DQ058602; AAY83877.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF226658; AAN40707.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_001013620.2; -.

UniGene

Bt.36568

3D structure databases

ModBase

Q865R1.

Ontologies

GO:0046592;	Molecular function: polyamine oxidase activity (inferred from electronic annotation from EC).
QuickGo view.

Family and domain databases

InterPro

IPR002937; Amino_oxidase.
Graphical view of domain structure.

Pfam

PF01593; Amino_oxidase; 1.
Pfam graphical view of domain structure.

BLOCKS

Q865R1.

Genome annotation databases

Ensembl

ENSBTAG00000018321; Bos taurus. [Contig view]

GeneID

282639; -.

Phylogenomic databases

HOVERGEN

Q865R1; -.

Other

ProtoNet

Q865R1.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cytoplasm; Direct protein sequencing; FAD; Flavoprotein; Oxidoreductase; Peroxisome.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`PROPEP`	`1 6`	`6`		`PRO_0000236802`
`CHAIN`	`7 512`	`506`	`Peroxisomal N(1)-acetyl-spermine/spermidine oxidase.`	`PRO_0000099874`
`MOTIF`	`510 512`	`3`	`Microbody targeting signal (Potential).`
`CONFLICT`	`7 7`		`Q -> E (in Ref. 2; AA sequence).`
`CONFLICT`	`482 482`		`T -> P (in Ref. 2; AAN40707).`

Sequence information

Length: 512 AA [This is the length of the unprocessed precursor]

Molecular weight: 56325 Da [This is the MW of the unprocessed precursor]

CRC64: 6164E3A77841F36F [This is a checksum on the sequence]

        10         20         30         40         50         60 
MQSGGRQAEA PGRGPRVLVV GGGIAGLGAA QRLCRHPAFS HLRVLEATAR AGGRIRSEHS 

        70         80         90        100        110        120 
FGGVVEVGAH WIHGPSQGNP VFQLAAKYGL LGEKALSEEN QLIETGGHVG LPSVSYASSG 

       130        140        150        160        170        180 
VSVSLELVAE MASLFYSLID QTREFLQAAE TTPPSVGEYL KEKIRQHMAG WTEDEETKKL 

       190        200        210        220        230        240 
KLAILKNLFN VECCVSGTHS MDLVALAPFG EYTVLPGLDC TFPEGYQGLT DCIMASLPKD 

       250        260        270        280        290        300 
VMVFDKPVKT IHWNGSFREA SAPGETFPVL VECEDGDCFP AHHVVVTVPL GFFKKHLDTF 

       310        320        330        340        350        360 
FEPPLPTEKV EAIRKIGFGT NNKIFLEFEE PFWEPDCQHI QVVWEDMSPL EDTAPELQDA 

       370        380        390        400        410        420 
WFKKLIGFWV LPPFQASHVL CGFIAGLESE FMETLSDEDV LRSLTQVLRR VTGNPQLPAP 

       430        440        450        460        470        480 
RSMLRSCWHS APYTRGSYSY VAVGSSGDDM DRLAQPLPSD GKGAQLQVLF AGEATHRTFY 

       490        500        510 
STTHGALLSG WREADRLMTL WDPQAQWPEP RL

Q865R1 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools