NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION, AND MASS SPECTROMETRY.
STRAIN=DSMZ 98-849;
DOI=10.1042/BJ20021047; PubMed=12379146 [NCBI, ExPASy, EBI, Israel, Japan]
Straganz G.D., Glieder A., Brecker L., Ribbons D.W., Steiner W.;
"Acetylacetone-cleaving enzyme Dke1: a novel C-C-bond-cleaving enzyme from Acinetobacter johnsonii.";
Biochem. J. 369:573-581(2003).

Comments

FUNCTION: Cleaves acetylacetone to equimolar amounts of methylglyoxal and acetate, consuming one equivalent of molecular oxygen.
CATALYTIC ACTIVITY: Pentane-2,4-dione + O₂ = acetate + 2-oxopropanal.
COFACTOR: Binds 1 iron ion per subunit.
PATHWAY: Xenobiotic degradation; acetylacetone degradation .
SUBUNIT: Homotetramer.
MASS SPECTROMETRY: Mass=16607; Method=MALDI; Range=1-153; Source=PubMed:12379146;.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AF489107; AAN45859.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

PDB

3BAL; X-ray; 1.95 A; A/B/C/D=1-153.

[ExPASy / RCSB / EBI]

PDBsum

3BAL; -.

ModBase

Q8GNT2.

Ontologies

GO:0033752;	Molecular function: acetylacetone-cleaving enzyme activity (inferred from electronic annotation from EC).
QuickGo view.

Family and domain databases

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

3D-structure; Dioxygenase; Direct protein sequencing; Iron; Metal-binding; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`		`Description`	`FTId`
`CHAIN`	`1 153`	`153`		`Acetylacetone-cleaving enzyme.`	`PRO_0000079925`

Sequence information

Length: 153 AA [This is the length of the unprocessed precursor]

Molecular weight: 16607 Da [This is the MW of the unprocessed precursor]

CRC64: F3FBCB762E8F250F [This is a checksum on the sequence]

        10         20         30         40         50         60 
MDYCNKKHTA EEYVKISDNN YVPFPEAFSD GGITWQLLHS SPETSSWTAI FNCPAGSSFA 

        70         80         90        100        110        120 
SHIHAGPGEY FLTKGKMEVR GGEQEGGSTA YAPSYGFESS GALHGKTFFP VESQFYMTFL 

       130        140        150 
GPLNFIDDNG KVIASIGWAE AQGAWLATKN EAA

Q8GNT2 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools