NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1016/S0140-6736(02)08713-5; PubMed=12044378 [NCBI, ExPASy, EBI, Israel, Japan]
Baba T., Takeuchi F., Kuroda M., Yuzawa H., Aoki K., Oguchi A., Nagai Y., Iwama N., Asano K., Naimi T., Kuroda H., Cui L., Yamamoto K., Hiramatsu K.;
"Genome and virulence determinants of high virulence community-acquired MRSA.";
Lancet 359:1819-1827(2002).

Comments

FUNCTION: May play a role in cell wall synthesis as L-alanine is an important constituent of the peptidoglycan layer (By similarity).
CATALYTIC ACTIVITY: L-alanine + H₂O + NAD⁺ = pyruvate + NH₃ + NADH.
PATHWAY: Amino-acid degradation; L-alanine degradation via dehydrogenase pathway; NH(3) and pyruvate from L-alanine: step 1/1 .
SIMILARITY: Belongs to the AlaDH/PNT family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

BA000033; BAB95517.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_646469.1; -.

3D structure databases

HSSP

O52942; 1SAY. [HSSP ENTRY / PDB]

ModBase

Q8NW54.

Enzyme and pathway databases

BioCyc

SAUR196620:MW1652-MON; -.

Family and domain databases

InterPro

IPR007698; Ala_DHase/PNT_C.
IPR008142; Ala_DHase/PNT_CS1.
IPR008143; Ala_DHase/PNT_CS2.
IPR007886; Ala_DHase/PNT_N.
IPR008141; Ala_DHase_PNT.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.50.720; NAD(P)-bd; 1.

Pfam

PF01262; AlaDh_PNT_C; 1.
PF05222; AlaDh_PNT_N; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR00518; alaDH; 1.

PROSITE

PS00836; ALADH_PNT_1; 1.
PS00837; ALADH_PNT_2; 1.

BLOCKS

Q8NW54.

Genome annotation databases

GeneID

1003764; -.

GenomeReviews

BA000033_GR; MW1652.

KEGG

sam:MW1652; -.

Phylogenomic databases

HOGENOM

Q8NW54; -.

Genome annotation databases

CMR

Q8NW54; MW1652.

Other

ProtoNet

Q8NW54.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; NAD; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 372`	`372`	`Alanine dehydrogenase 2.`	`PRO_0000199006`
`NP_BIND`	`169 199`	`31`	`NAD (By similarity).`
`ACT_SITE`	`95 95`		`Potential.`

Sequence information

Length: 372 AA [This is the length of the unprocessed precursor]

Molecular weight: 40105 Da [This is the MW of the unprocessed precursor]

CRC64: 32BA3DA4734EC9FB [This is a checksum on the sequence]

        10         20         30         40         50         60 
MKIGIPREIK NNENRVGLSP SGVHALVESG HTVLVETNAG SGSFFEDVDY KEAGAEIVAE 

        70         80         90        100        110        120 
QAKVWDVDMV IKVKEPLESE YPYFKEGLVL FTYLHLANEE KLTQALIDRK VISIAYETVQ 

       130        140        150        160        170        180 
LPDRSLPLLS PMSEVAGRMS AQVGAEFLQK LNGGMGILLG GVPGVPKGKV TIIGGGQAGT 

       190        200        210        220        230        240 
NAAKIALGLG ADVTILDVNP KRLQQLDDLF GGRVHTIMSN PLNIELYVKQ SDLVIGAVLI 

       250        260        270        280        290        300 
PGAKAPRLVT EDMIKQMKNG SVIIDIAIDQ GGIFETTDKI TTHDDPTYIK HGVVHYAVAN 

       310        320        330        340        350        360 
MPGAVPRTST LALNNATLPY ALMLANKGYR EAFKSNQPLS LGLNTYKGHV TNKGVAEAFE 

       370 
MEYKSVEEAL QL

Q8NW54 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools