ID   NAPA_YERPE              Reviewed;         830 AA.
AC   Q8ZCF3; Q0WCN0; Q74SF1;
DT   07-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   25-NOV-2008, entry version 46.
DE   RecName: Full=Periplasmic nitrate reductase;
DE            EC=1.7.99.4;
DE   Flags: Precursor;
GN   Name=napA; OrderedLocusNames=YPO3038, YP_2661;
OS   Yersinia pestis.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Yersinia.
OX   NCBI_TaxID=632;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CO-92 / Biovar Orientalis;
RX   MEDLINE=21470413; PubMed=11586360; DOI=10.1038/35097083;
RA   Parkhill J., Wren B.W., Thomson N.R., Titball R.W., Holden M.T.G.,
RA   Prentice M.B., Sebaihia M., James K.D., Churcher C.M., Mungall K.L.,
RA   Baker S., Basham D., Bentley S.D., Brooks K., Cerdeno-Tarraga A.-M.,
RA   Chillingworth T., Cronin A., Davies R.M., Davis P., Dougan G.,
RA   Feltwell T., Hamlin N., Holroyd S., Jagels K., Karlyshev A.V.,
RA   Leather S., Moule S., Oyston P.C.F., Quail M.A., Rutherford K.M.,
RA   Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G.;
RT   "Genome sequence of Yersinia pestis, the causative agent of plague.";
RL   Nature 413:523-527(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=91001 / Biovar Mediaevalis;
RX   PubMed=15368893; DOI=10.1093/dnares/11.3.179;
RA   Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D.,
RA   Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z.,
RA   Jin L., Dai R., Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J.,
RA   Yang H., Wang J., Huang P., Yang R.;
RT   "Complete genome sequence of Yersinia pestis strain 91001, an isolate
RT   avirulent to humans.";
RL   DNA Res. 11:179-197(2004).
CC   -!- FUNCTION: Catalytic subunit of the periplasmic nitrate reductase
CC       (NAP). Only expressed at high levels during aerobic growth. NapAB
CC       complex receives electrons from the membrane-anchored tetraheme
CC       protein napC, thus allowing electron flow between membrane and
CC       periplasm. Essential function for nitrate assimilation and may
CC       have a role in anaerobic metabolism (By similarity).
CC   -!- CATALYTIC ACTIVITY: Nitrite + acceptor = nitrate + reduced
CC       acceptor.
CC   -!- COFACTOR: Binds 1 4Fe-4S cluster (By similarity).
CC   -!- COFACTOR: Binds 1 molybdenum ion per subunit (By similarity).
CC   -!- COFACTOR: Binds 2 molybdopterin guanine dinucleotide (MGD) groups
CC       per subunit (By similarity).
CC   -!- SUBUNIT: Interacts with napB (By similarity).
CC   -!- SUBCELLULAR LOCATION: Periplasm (By similarity).
CC   -!- PTM: Predicted to be exported by the Tat system. The position of
CC       the signal peptide cleavage has not been experimentally proven.
CC   -!- SIMILARITY: Belongs to the prokaryotic molybdopterin-containing
CC       oxidoreductase family. NasA/napA/narB subfamily.
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DR   EMBL; AL590842; CAL21640.1; -; Genomic_DNA.
DR   EMBL; AE017042; AAS62853.1; -; Genomic_DNA.
DR   PIR; AE0369; AE0369.
DR   RefSeq; NP_993976.1; -.
DR   HSSP; Q53176; 1OGY.
DR   SMR; Q8ZCF3; 38-827.
DR   GeneID; 2766408; -.
DR   GenomeReviews; AE017042_GR; YP_2661.
DR   GenomeReviews; AL590842_GR; YPO3038.
DR   KEGG; ype:YPO3038; -.
DR   KEGG; ypm:YP_2661; -.
DR   HOGENOM; Q8ZCF3; -.
DR   BioCyc; YPES214092:YPO3038-MON; -.
DR   BioCyc; YPES229193:YP2661-MON; -.
DR   GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-KW.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:InterPro.
DR   GO; GO:0009055; F:electron carrier activity; IEA:HAMAP.
DR   GO; GO:0005506; F:iron ion binding; IEA:HAMAP.
DR   GO; GO:0030151; F:molybdenum ion binding; IEA:InterPro.
DR   GO; GO:0008940; F:nitrate reductase activity; IEA:HAMAP.
DR   GO; GO:0022900; P:electron transport chain; IEA:UniProtKB-KW.
DR   GO; GO:0006777; P:Mo-molybdopterin cofactor biosynthetic process; IEA:HAMAP.
DR   GO; GO:0042128; P:nitrate assimilation; IEA:HAMAP.
DR   GO; GO:0006810; P:transport; IEA:UniProtKB-KW.
DR   HAMAP; MF_01630; -; 1.
DR   InterPro; IPR009010; Asp_de-COase-like_fold.
DR   InterPro; IPR006656; Mopterin_OxRdtase.
DR   InterPro; IPR006963; Mopterin_OxRdtase_Fe4S4.
DR   InterPro; IPR006655; Mopterin_OxRdtase_prok_CS.
DR   InterPro; IPR006657; MPT_dinuc_bd.
DR   InterPro; IPR010051; NO3_reductase_lsu_periplasm.
DR   InterPro; IPR006311; Tat.
DR   Gene3D; G3DSA:2.40.40.20; Asp_decarboxylase-like_fold; 1.
DR   Pfam; PF04879; Molybdop_Fe4S4; 1.
DR   Pfam; PF00384; Molybdopterin; 1.
DR   Pfam; PF01568; Molydop_binding; 1.
DR   TIGRFAMs; TIGR01706; NAPA; 1.
DR   TIGRFAMs; TIGR01409; TAT_signal_seq; 1.
DR   PROSITE; PS00551; MOLYBDOPTERIN_PROK_1; 1.
DR   PROSITE; PS00490; MOLYBDOPTERIN_PROK_2; FALSE_NEG.
DR   PROSITE; PS00932; MOLYBDOPTERIN_PROK_3; FALSE_NEG.
DR   PROSITE; PS51318; TAT; 1.
PE   3: Inferred from homology;
KW   4Fe-4S; Complete proteome; Electron transport; Iron; Iron-sulfur;
KW   Metal-binding; Molybdenum; Nitrate assimilation; Oxidoreductase;
KW   Periplasm; Signal; Transport.
FT   SIGNAL        1     31       Tat-type signal (Potential).
FT   CHAIN        32    830       Periplasmic nitrate reductase.
FT                                /FTId=PRO_0000046015.
FT   METAL        46     46       Iron-sulfur (4Fe-4S) (By similarity).
FT   METAL        49     49       Iron-sulfur (4Fe-4S) (By similarity).
FT   METAL        53     53       Iron-sulfur (4Fe-4S) (By similarity).
FT   METAL        81     81       Iron-sulfur (4Fe-4S) (By similarity).
FT   CONFLICT    341    341       A -> T (in Ref. 2; AAS62853).
SQ   SEQUENCE   830 AA;  93226 MW;  C83E1665F370EF6D CRC64;
     MKLSRRDFMK ANAAVAAAAA AGMTIPTVAK AVGETTNAIK WDKAPCRFCG TGCGVLVGTQ
     NGRIVASQGD PDSPVNRGLN CIKGYFLPKI MYGKDRLTQP LLRMKDGQYD KEGDFTPISW
     EKAFDIMELK FKNALKEKGP TAVGMFGSGQ WTVWEGYAAL KLLKGGFRSN NLDPNARHCM
     ASSVVGFMRT FGMDEPMGCY DDIEEADAFV LWGSNMAEMH PVLWSRMTSR RLTNAHVRIA
     VLSTYEHRSF ELADNPIVFT PQTDLVIMNY IANYIIQNNA VDKDFLAQHV NFRRGATDIG
     YGLRPTHPLE KAAKNPGSDA SEPMSFEDFK TFVAEYTLEK AAKMSGVPED QLESLAQLYA
     DPKVKLVSYW TMGFNQHTRG VWANNMCYNL HLLTGKISTP GSGPFSLTGQ PSACGTAREV
     GTFSHRLPAD MVVTNEKHRQ IAETTWQLPA GTIPEKVGLH AVAQDRALKD GTLNAYWVMC
     NNNMQAGPNI NEERMPGWRD PRNFIVVSDP YPTISALSAD LILPTSMWVE KEGAYGNAER
     RTQFWRQQVP SPGEAKSDLW QIVEFAKRFN VEEVWPAELV NQKPEYRGKN LYEVLFANDV
     VSKYPLSEIP DDQLNDEARD FGFYIQKGLF EEYASFGRGH AHDLAPFDVY HQVRGLRWPV
     VDGKETLWRY REGFDPFVPK GEEVRFYGKP DGKAVIFALP YEPAAESPDQ EYDLWLSTGR
     VLEHWHTGSM TRRVPELHRA FPEAVLFIHP LDAKARGLHR GDKVKVISRR GEVISLVETR
     GRNRPPRGLV YMPFFDAAQL VNNLTLDATD PLSKETDFKK CAVKLERVVA
//