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UniProtKB/Swiss-Prot entry Q93ZB2


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name KO1_ARATH
Primary accession number Q93ZB2
Secondary accession numbers O81188 Q9S7C8
Integrated into Swiss-Prot on July 19, 2004
Sequence was last modified on July 19, 2004 (Sequence version 2)
Annotations were last modified on    September 2, 2008 (Entry version 44)
Name and origin of the protein
Protein name Ent-kaurene oxidase
Synonyms AtKO1
EC 1.14.13.78
Cytochrome P450 701A3
Gene name
Name: KO1
Synonyms: CYP701A3, GA3
OrderedLocusNames: At5g25900
ORFNames: T1N24.23
From
Arabidopsis thaliana (Mouse-ear cress) [TaxID: 3702] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, ENZYME ACTIVITY, AND TISSUE SPECIFICITY.
STRAIN=cv. Columbia, and cv. Landsberg erecta;
TISSUE=Silique;
DOI=10.1073/pnas.95.15.9019; PubMed=9671797 [NCBI, ExPASy, EBI, Israel, Japan]
Helliwell C.A., Sheldon C.C., Olive M.R., Walker A.R., Zeevaart J.A.D., Peacock W.J., Dennis E.S.;
"Cloning of the Arabidopsis ent-kaurene oxidase gene GA3.";
Proc. Natl. Acad. Sci. U.S.A. 95:9019-9024(1998).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Columbia;
DOI=10.1038/35048507; PubMed=11130714 [NCBI, ExPASy, EBI, Israel, Japan]
Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K., Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.;
"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
Nature 408:823-826(2000).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=cv. Columbia;
DOI=10.1126/science.1088305; PubMed=14593172 [NCBI, ExPASy, EBI, Israel, Japan]
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
"Empirical analysis of transcriptional activity in the Arabidopsis genome.";
Science 302:842-846(2003).
[4]
CATALYTIC ACTIVITY.
DOI=10.1104/pp.119.2.507; PubMed=9952446 [NCBI, ExPASy, EBI, Israel, Japan]
Helliwell C.A., Poole A., Peacock W.J., Dennis E.S.;
"Arabidopsis ent-kaurene oxidase catalyzes three steps of gibberellin biosynthesis.";
Plant Physiol. 119:507-510(1999).
[5]
SUBCELLULAR LOCATION.
PubMed=11722763 [NCBI, ExPASy, EBI, Israel, Japan]
Helliwell C.A., Sullivan J.A., Mould R.M., Gray J.C., Peacock W.J., Dennis E.S.;
"A plastid envelope location of Arabidopsis ent-kaurene oxidase links the plastid and endoplasmic reticulum steps of the gibberellin biosynthesis pathway.";
Plant J. 28:201-208(2001).
[6]
TISSUE SPECIFICITY.
PubMed=11737781 [NCBI, ExPASy, EBI, Israel, Japan]
Yamaguchi S., Kamiya Y., Sun T.-P.;
"Distinct cell-specific expression patterns of early and late gibberellin biosynthetic genes during Arabidopsis seed germination.";
Plant J. 28:443-453(2001).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AF047719; AAC39505.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF047720; AAC39506.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF047721; AAC39507.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF149413; AAD40137.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY057671; AAL15302.1; ALT_FRAME; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR T51806; T51806.
RefSeq NP_197962.1; -.
UniGene At.10791
3D structure databases
ModBase Q93ZB2.
Organism-specific databases
GeneFarm 1435; 94.
TAIR At5g25900; -.
Gene expression databases
ArrayExpress Q93ZB2; -.
GermOnline AT5G25900; Arabidopsis thaliana.
Family and domain databases
InterPro IPR001128; Cyt_P450.
IPR002401; Cyt_P450_E_grp-I.
Graphical view of domain structure.
Gene3D G3DSA:1.10.630.10; Cyt_P450; 1.
PANTHER PTHR19383; Cyt_P450; 1.
Pfam PF00067; p450; 1.
Pfam graphical view of domain structure.
PRINTS PR00463; EP450I.
PR00385; P450.
PROSITE PS00086; CYTOCHROME_P450; 1.
BLOCKS Q93ZB2.
Genome annotation databases
GeneID 832659; -.
GenomeReviews BA000015_GR; AT5G25900.
KEGG ath:AT5G25900; -.
NMPDR fig|3702.1.peg.24790; -.
Other
ProtoNet Q93ZB2.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Chloroplast; Complete proteome; Heme; Iron; Membrane; Metal-binding; Monooxygenase; NADP; Oxidoreductase; Plastid; Plastid outer membrane; Transmembrane.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   509  509     Ent-kaurene oxidase. PRO_0000052181
TOPO_DOM   1     2  2     Chloroplast intermembrane (Potential). 
TRANSMEM   3    23  21     Potential. 
TOPO_DOM   24   509  486     Cytoplasmic (Potential). 
METAL   454   454        Iron (heme axial ligand) (By similarity). 
CONFLICT   97    97        A -> G (in Ref. 1; AAC39507). 
Sequence information
Length: 509 AA [This is the length of the unprocessed precursor] Molecular weight: 58160 Da [This is the MW of the unprocessed precursor] CRC64: A84F7E2C78F0E83F [This is a checksum on the sequence]
        10         20         30         40         50         60 
MAFFSMISIL LGFVISSFIF IFFFKKLLSF SRKNMSEVST LPSVPVVPGF PVIGNLLQLK 

        70         80         90        100        110        120 
EKKPHKTFTR WSEIYGPIYS IKMGSSSLIV LNSTETAKEA MVTRFSSIST RKLSNALTVL 

       130        140        150        160        170        180 
TCDKSMVATS DYDDFHKLVK RCLLNGLLGA NAQKRKRHYR DALIENVSSK LHAHARDHPQ 

       190        200        210        220        230        240 
EPVNFRAIFE HELFGVALKQ AFGKDVESIY VKELGVTLSK DEIFKVLVHD MMEGAIDVDW 

       250        260        270        280        290        300 
RDFFPYLKWI PNKSFEARIQ QKHKRRLAVM NALIQDRLKQ NGSESDDDCY LNFLMSEAKT 

       310        320        330        340        350        360 
LTKEQIAILV WETIIETADT TLVTTEWAIY ELAKHPSVQD RLCKEIQNVC GGEKFKEEQL 

       370        380        390        400        410        420 
SQVPYLNGVF HETLRKYSPA PLVPIRYAHE DTQIGGYHVP AGSEIAINIY GCNMDKKRWE 

       430        440        450        460        470        480 
RPEDWWPERF LDDGKYETSD LHKTMAFGAG KRVCAGALQA SLMAGIAIGR LVQEFEWKLR 

       490        500 
DGEEENVDTY GLTSQKLYPL MAIINPRRS 

Q93ZB2 in FASTA format

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