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UniProtKB/Swiss-Prot entry Q95JH5

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name AK1C4_MACFA
Primary accession number Q95JH5
Secondary accession numbers None
Integrated into Swiss-Prot on June 7, 2005
Sequence was last modified on December 1, 2001 (Sequence version 1)
Annotations were last modified on    September 2, 2008 (Entry version 35)
Name and origin of the protein
Protein name Aldo-keto reductase family 1 member C4
Synonyms EC 1.1.1.-
Chlordecone reductase
CDR
EC 1.1.1.225
3-alpha-hydroxysteroid dehydrogenase type I
EC 1.1.1.50
3-alpha-HSD1
Dihydrodiol dehydrogenase 4
DD-4
DD4
Gene name
Name: AKR1C4
From
Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey) [TaxID: 9541] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Cercopithecidae; Cercopithecinae; Macaca.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 2-23; 69-116; 137-153; 184-246 AND 250-323.
TISSUE=Liver;
DOI=10.2133/dmpk.17.348; PubMed=15618685 [NCBI, ExPASy, EBI, Israel, Japan]
Higaki Y., Kamiya T., Usami N., Shintani S., Shiraishi H., Ishikura S., Yamamoto I., Hara A.;
"Molecular characterization of two monkey dihydrodiol dehydrogenases.";
Drug Metab. Pharmacokinet. 17:348-356(2002).
Comments
  • FUNCTION: Catalyzes the transformation of the potent androgen dihydrotestosterone (DHT) into the less active form, 5-alpha-androstan-3-alpha,17-beta-diol (3-alpha-diol). Also has some 20-alpha-hydroxysteroid dehydrogenase activity. The biotransformation of the pesticide chlordecone (kepone) to its corresponding alcohol leads to increased biliary excretion of the pesticide and concomitant reduction of its neurotoxicity since bile is the major excretory route.
  • CATALYTIC ACTIVITY: Chlordecone alcohol + NADP+ = chlordecone + NADPH.
  • CATALYTIC ACTIVITY: Androsterone + NAD(P)+ = 5-alpha-androstane-3,17-dione + NAD(P)H.
  • SUBUNIT: Monomer (By similarity).
  • SUBCELLULAR LOCATION: Cytoplasm (By similarity).
  • SIMILARITY: Belongs to the aldo/keto reductase family.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AB070211; BAB63208.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
3D structure databases
HSSP Q04828; 1MRQ. [HSSP ENTRY / PDB]
SMR Q95JH5; 1-323.
ModBase Q95JH5.
Family and domain databases
InterPro IPR001395; Aldo/ket_red.
Graphical view of domain structure.
Gene3D G3DSA:3.20.20.100; Aldo/ket_red; 1.
PANTHER PTHR11732; Aldo/ket_red; 1.
Pfam PF00248; Aldo_ket_red; 1.
Pfam graphical view of domain structure.
PRINTS PR00069; ALDKETRDTASE.
ProDom PD000288; Aldo/ket_red; 1.
[Domain structure / List of seq. sharing at least 1 domain]
PROSITE PS00798; ALDOKETO_REDUCTASE_1; 1.
PS00062; ALDOKETO_REDUCTASE_2; 1.
PS00063; ALDOKETO_REDUCTASE_3; 1.
BLOCKS Q95JH5.
Phylogenomic databases
HOVERGEN Q95JH5; -.
Other
ProtoNet Q95JH5.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Cytoplasm; Direct protein sequencing; NADP; Oxidoreductase; Phosphoprotein.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   323  323     Aldo-keto reductase family 1 member C4. PRO_0000124641
NP_BIND   13    22  10     NADP (Potential). 
NP_BIND   217   280  64     NADP (By similarity). 
ACT_SITE   55    55        Proton donor (By similarity). 
BINDING   117   117        Substrate (By similarity). 
SITE   54    54  1     Important for substrate specificity (By similarity). 
SITE   84    84  1     Lowers pKa of active site Tyr (By similarity). 
MOD_RES   196   196        Phosphotyrosine (By similarity). 
Sequence information
Length: 323 AA [This is the length of the unprocessed precursor] Molecular weight: 37117 Da [This is the MW of the unprocessed precursor] CRC64: 3E7F2E78815AA196 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MDPKYQRVAL NDGHFMPVLG FGTYAPPEVP RNRVVEVTKL AIEAGFRHID SAYLYNNEEQ 

        70         80         90        100        110        120 
VGLAIRSKIA DGSVKREDIF YTSKLWCTFF RPQLVQPALE SSLKKLQLDY VDLYLIHFPM 

       130        140        150        160        170        180 
ALKPGETPLP KDENGKVMFD TVDLCAIWEA MEKCKDAGLA KSIGVSNFNR RQLEMILNNP 

       190        200        210        220        230        240 
GLKYKPVCNQ VECHPYLNQS KLLDFCKSKD IVLVAHSALG TQRHKLWVDQ NSPALLEDPV 

       250        260        270        280        290        300 
LCALAKKHKR SPALIALRYQ LQRGVVVLAK SYNEQRIREN VQVFEFQLTS EDMKVLDDLN 

       310        320 
RNFRYVVMDF LVDHPDYPFS DEY
Q95JH5 in FASTA format

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