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UniProtKB/Swiss-Prot entry Q96330


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name FLS1_ARATH
Primary accession number Q96330
Secondary accession numbers O04730 O04731 O04732 O04830 O04831 O04832
Integrated into Swiss-Prot on July 15, 1999
Sequence was last modified on February 1, 1997 (Sequence version 1)
Annotations were last modified on    September 2, 2008 (Entry version 61)
Name and origin of the protein
Protein name Flavonol synthase/flavanone 3-hydroxylase
Synonyms EC 1.14.11.23
EC 1.14.11.9
FLS 1
Gene name
Name: FLS1
OrderedLocusNames: At5g08640
ORFNames: MAH20.20
From
Arabidopsis thaliana (Mouse-ear cress) [TaxID: 3702] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=cv. Landsberg erecta;
DOI=10.1104/pp.113.4.1437; PubMed=9112784 [NCBI, ExPASy, EBI, Israel, Japan]
Pelletier M.K., Murrell J.R., Shirley B.W.;
"Characterization of flavonol synthase and leucoanthocyanidin dioxygenase genes in Arabidopsis. Further evidence for differential regulation of 'early' and 'late' genes.";
Plant Physiol. 113:1437-1445(1997).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
STRAIN=cv. Columbia, and cv. Landsberg erecta;
DOI=10.1073/pnas.95.21.12432; PubMed=9770503 [NCBI, ExPASy, EBI, Israel, Japan]
Wisman E., Hartmann U., Sagasser M., Baumann E., Palme K., Hahlbrock K., Saedler H., Weisshaar B.;
"Knock-out mutants from an En-1 mutagenized Arabidopsis thaliana population generate phenylpropanoid biosynthesis phenotypes.";
Proc. Natl. Acad. Sci. U.S.A. 95:12432-12437(1998).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Columbia;
DOI=10.1093/dnares/4.4.291; PubMed=9405937 [NCBI, ExPASy, EBI, Israel, Japan]
Kotani H., Nakamura Y., Sato S., Kaneko T., Asamizu E., Miyajima N., Tabata S.;
"Structural analysis of Arabidopsis thaliana chromosome 5. II. Sequence features of the regions of 1,044,062 bp covered by thirteen physically assigned P1 clones.";
DNA Res. 4:291-300(1997).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=cv. Columbia;
DOI=10.1126/science.1088305; PubMed=14593172 [NCBI, ExPASy, EBI, Israel, Japan]
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
"Empirical analysis of transcriptional activity in the Arabidopsis genome.";
Science 302:842-846(2003).
[5]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.;
"Full-length cDNA from Arabidopsis thaliana.";
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
U72631; AAB17393.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U84258; AAC69362.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U84259; AAC69363.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U84260; AAB41504.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AB006697; BAB10013.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY058068; AAL24176.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BT000494; AAN18063.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY086328; AAM64397.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_196481.1; -.
UniGene At.8771
3D structure databases
HSSP Q96323; 1GP6. [HSSP ENTRY / PDB]
ModBase Q96330.
Organism-specific databases
TAIR At5g08640; -.
Gene expression databases
ArrayExpress Q96330; -.
GermOnline AT5G08640; Arabidopsis thaliana.
Family and domain databases
InterPro IPR005123; 2OG-FeII_Oase.
Graphical view of domain structure.
Pfam PF03171; 2OG-FeII_Oxy; 1.
Pfam graphical view of domain structure.
BLOCKS Q96330.
Proteomic databases
ProMEX Q96330; -.
Genome annotation databases
GeneID 830765; -.
GenomeReviews BA000015_GR; AT5G08640.
KEGG ath:AT5G08640; -.
NMPDR fig|3702.1.peg.23017; -.
Other
ProtoNet Q96330.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Cytoplasm; Dioxygenase; Flavonoid biosynthesis; Iron; Metal-binding; Oxidoreductase; Vitamin C.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   336  336     Flavonol synthase/flavanone 3-hydroxylase. PRO_0000067291
METAL   221   221        Iron (By similarity). 
METAL   223   223        Iron (By similarity). 
METAL   277   277        Iron (By similarity). 
Sequence information
Length: 336 AA [This is the length of the unprocessed precursor] Molecular weight: 38282 Da [This is the MW of the unprocessed precursor] CRC64: 3283E3AFE603D2A9 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MEVERVQDIS SSSLLTEAIP LEFIRSEKEQ PAITTFRGPT PAIPVVDLSD PDEESVRRAV 

        70         80         90        100        110        120 
VKASEEWGLF QVVNHGIPTE LIRRLQDVGR KFFELPSSEK ESVAKPEDSK DIEGYGTKLQ 

       130        140        150        160        170        180 
KDPEGKKAWV DHLFHRIWPP SCVNYRFWPK NPPEYREVNE EYAVHVKKLS ETLLGILSDG 

       190        200        210        220        230        240 
LGLKRDALKE GLGGEMAEYM MKINYYPPCP RPDLALGVPA HTDLSGITLL VPNEVPGLQV 

       250        260        270        280        290        300 
FKDDHWFDAE YIPSAVIVHI GDQILRLSNG RYKNVLHRTT VDKEKTRMSW PVFLEPPREK 

       310        320        330 
IVGPLPELTG DDNPPKFKPF AFKDYSYRKL NKLPLD 

Q96330 in FASTA format

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