FUNCTION: Catalyzes the reduction of the double bond of an array of alpha, beta-unsaturated aldehydes and ketones. It also reduces the nitro group of nitroester and nitroaromatic compounds. It could have a role in detoxification processes (By similarity).
CATALYTIC ACTIVITY: NADPH + acceptor = NADP⁺ + reduced acceptor.
COFACTOR: FMN (By similarity).
SUBUNIT: Homotetramer (By similarity).
SIMILARITY: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family. NamA subfamily.

Copyright

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Cross-references

Sequence databases

EMBL

AE001437; AAK81164.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

A97297; A97297.

RefSeq

NP_349824.1; -.

3D structure databases

HSSP

Q02899; 1OYC. [HSSP ENTRY / PDB]

ModBase

Q97E86.

Enzyme and pathway databases

BioCyc

CACE272562:CAC3229-MON; -.

Ontologies

GO:0003959;	Molecular function: NADPH dehydrogenase activity (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_01614; -; 1.
PBIL [Tree]

InterPro

IPR013785; Aldolase_TIM.
IPR001155; OxRdtase_FMN_N.
Graphical view of domain structure.

Gene3D

G3DSA:3.20.20.70; Aldolase_TIM; 1.

Pfam

PF00724; Oxidored_FMN; 1.
Pfam graphical view of domain structure.

BLOCKS

Q97E86.

Genome annotation databases

GeneID

1119411; -.

GenomeReviews

AE001437_GR; CA_C3229.

KEGG

cac:CAC3229; -.

NMPDR

fig|272562.1.peg.3353; -.

Phylogenomic databases

HOGENOM

Q97E86; -.

Genome annotation databases

CMR

Q97E86; CA_C3229.

Other

ProtoNet

Q97E86.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; Flavoprotein; FMN; NADP; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 339`	`339`	`NADPH dehydrogenase.`	`PRO_0000216120`
`NP_BIND`	`21 25`	`5`	`FMN (By similarity).`
`BINDING`	`26 26`		`Substrate (By similarity).`
`BINDING`	`162 162`		`Substrate (By similarity).`
`BINDING`	`165 165`		`Substrate (By similarity).`
`BINDING`	`215 215`		`FMN (By similarity).`
`BINDING`	`308 308`		`FMN (By similarity).`

Sequence information

Length: 339 AA [This is the length of the unprocessed precursor]

Molecular weight: 37677 Da [This is the MW of the unprocessed precursor]

CRC64: 5958453FA93F2EDF [This is a checksum on the sequence]

        10         20         30         40         50         60 
MKIFEAYTIK NMCLKNRIVM PPMCMYSSDN TGNINDFHLV HYTTRSIGGV GFIIVEATGI 

        70         80         90        100        110        120 
TPNGRISDKD LGIWSEKHAE GLSFLVKEVK KYGSKIAIQL NHSGRKYEGT SGEPVAPSAL 

       130        140        150        160        170        180 
AFDENSKTPK ELTKNEIKEI ILAFKAAAKR AEKAGFDAIE IHGAHGYLIN QFLSPLSNFR 

       190        200        210        220        230        240 
DDEYGGSTEN RTRFLKEVLE AVREVWPKEK PILLRVSAED YRGGSGITPN EMVNIINIVK 

       250        260        270        280        290        300 
DLIDVVDVSS GGVAPAHINL YPGYQVKLSE VIKNECNVPT IAVGLICDIN MVEEILSNNR 

       310        320        330 
ADLVALGREL LRNPYFVLSS ARLKNINIDF PKPYERAFN

Q97E86 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools