[1]	NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY. STRAIN=Wistar; TISSUE=Brain; DOI=10.1006/bbrc.2001.5997; PubMed=11716481 [NCBI, ExPASy, EBI, Israel, Japan] Kitagawa K., Yagyu K., Yamamoto A., Hattori N., Omori K., Zeng X.-T., Inagaki C.; "Molecular cloning and characterization of the Cl(-) pump-associated 55-kDa protein in rat brain."; Biochem. Biophys. Res. Commun. 289:363-371(2001).
[2]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. TISSUE=Kidney; DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan] The MGC Project Team; "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."; Genome Res. 14:2121-2127(2004).
[3]	PROTEIN SEQUENCE OF 291-318 AND 420-429, AND MASS SPECTROMETRY. STRAIN=Sprague-Dawley; TISSUE=Brain; Lubec G., Kang S.U., Lubec S.; Submitted (SEP-2007) to UniProtKB.

Comments

FUNCTION: Involved in the degradation of prenylated proteins. Cleaves the thioether bond of prenyl-L-cysteines, such as farnesylcysteine and geranylgeranylcysteine (By similarity).
CATALYTIC ACTIVITY: An S-prenyl-L-cysteine + O₂ + H₂O = a prenal + L-cysteine + H₂O₂.
COFACTOR: FAD (By similarity).
SUBCELLULAR LOCATION: Lysosome (By similarity).
TISSUE SPECIFICITY: Expressed mainly in cerebrum.
SIMILARITY: Belongs to the prenylcysteine oxidase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AF332142; AAK16548.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BC078719; AAH78719.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

JC7782; JC7782.

NP_659553.1; -.

3D structure databases

ModBase

Q99ML5.

Organism-specific databases

RGD

628652; Pcyox1.

Gene expression databases

ArrayExpress

Q99ML5; -.

GermOnline

ENSRNOG00000016704; Rattus norvegicus.

Family and domain databases

InterPro

IPR013027; FAD_pyr_nucl-diS_OxRdtase.
IPR010795; Prenylcys_lyase.
IPR017046; Prenylcysteine_Oxase.
Graphical view of domain structure.

Pfam

PF07156; Prenylcys_lyase; 1.
PF07992; Pyr_redox_2; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF036292; Prenylcysteine_oxidase; 1.

BLOCKS

Q99ML5.

Genome annotation databases

Ensembl

ENSRNOG00000016704; Rattus norvegicus. [Contig view]

GeneID

246302; -.

KEGG

rno:246302; -.

Phylogenomic databases

HOVERGEN

Q99ML5; -.

Other

ProtoNet

Q99ML5.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Direct protein sequencing; FAD; Glycoprotein; Lysosome; Oxidoreductase; Signal; Ubl conjugation.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`SIGNAL`	`1 28`	`28`	`Potential.`
`CHAIN`	`29 504`	`476`	`Prenylcysteine oxidase.`	`PRO_0000023302`
`CARBOHYD`	`196 196`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`322 322`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`352 352`		`N-linked (GlcNAc...) (Potential).`
`CROSSLNK`	`162 162`		`Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) (By similarity).`

Sequence information

Length: 504 AA [This is the length of the unprocessed precursor]

Molecular weight: 56288 Da [This is the MW of the unprocessed precursor]

CRC64: D2778026128FE9D4 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MGRFAATLVG SLFGLGLLLC GLGRLASAEP RAPPEKIAIV GAGIGGTSSA YYLRKKFGKD 

        70         80         90        100        110        120 
VKIDVFEREE IGGRLATLKV QGHDYEAGGS VIHPLNLHMK RFVKELGLSS VPASGGLVGV 

       130        140        150        160        170        180 
YNGKSLVFEE SSWFIINVIK LVWRYGFQSL RMHMWVEDLL DKFMRIYRYQ SHDYAFSSVE 

       190        200        210        220        230        240 
KLMYAIGGDD YVRLLNQTLR ENLKKAGFSE TFLNEMIAPV MKVNFGQSTD LNAFVGAVSM 

       250        260        270        280        290        300 
TAADSNLWAV EGGNKVVCSG LLQASNSNLI SGSVMSIEKT RTKQTGTPPK MYEVVYKTGS 

       310        320        330        340        350        360 
EIHSDFYDIV LVAAPLNRKM SNITFRNFDP PIEEFNDPYQ QLVTTLIKGE LNSTLFSSRH 

       370        380        390        400        410        420 
KDQFGLSAIL VTDDSDMFIN SLSIVASVSH KEGPPPAVDG MHVWKTFSKD ILTKEQISKL 

       430        440        450        460        470        480 
FLSYDYAVRK PWLSYPYYNP PQKCPSIILH DRLYYLNGIE FAASCMEMSA IAGYNAALLA 

       490        500 
YHRWNGNEDM IDQDDLYEKL KTEL

Q99ML5 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools