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UniProtKB/Swiss-Prot entry Q9C5Y2


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name KAO2_ARATH
Primary accession number Q9C5Y2
Secondary accession number Q9ZV72
Integrated into Swiss-Prot on July 19, 2004
Sequence was last modified on July 19, 2004 (Sequence version 2)
Annotations were last modified on    July 22, 2008 (Entry version 46)
Name and origin of the protein
Protein name Ent-kaurenoic acid oxidase 2
Synonyms AtKAO2
EC 1.14.13.79
Cytochrome P450 88A4
Gene name
Name: KAO2
Synonyms: CYP88A4
OrderedLocusNames: At2g32440
ORFNames: T32F6.4
From
Arabidopsis thaliana (Mouse-ear cress) [TaxID: 3702] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
Protein existence 2: Evidence at transcript level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ENZYME ACTIVITY, AND TISSUE SPECIFICITY.
STRAIN=cv. Landsberg erecta;
TISSUE=Silique;
DOI=10.1073/pnas.041588998; PubMed=11172076 [NCBI, ExPASy, EBI, Israel, Japan]
Helliwell C.A., Chandler P.M., Poole A., Dennis E.S., Peacock J.W.;
"The CYP88A cytochrome P450, ent-kaurenoic acid oxidase, catalyzes three steps of the gibberellin biosynthesis pathway.";
Proc. Natl. Acad. Sci. U.S.A. 98:2065-2070(2001).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Columbia;
DOI=10.1038/45471; PubMed=10617197 [NCBI, ExPASy, EBI, Israel, Japan]
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
Nature 402:761-768(1999).
[3]
SUBCELLULAR LOCATION.
PubMed=11722763 [NCBI, ExPASy, EBI, Israel, Japan]
Helliwell C.A., Sullivan J.A., Mould R.M., Gray J.C., Peacock W.J., Dennis E.S.;
"A plastid envelope location of Arabidopsis ent-kaurene oxidase links the plastid and endoplasmic reticulum steps of the gibberellin biosynthesis pathway.";
Plant J. 28:201-208(2001).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AF318501; AAK11565.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AC005700; AAC69934.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR B84733; B84733.
RefSeq NP_180803.1; -.
UniGene At.13205
3D structure databases
HSSP P14779; 1JPZ. [HSSP ENTRY / PDB]
ModBase Q9C5Y2.
Enzyme and pathway databases
BioCyc MetaCyc:AT2G32440-MON; -.
Organism-specific databases
GeneFarm 1519; 94.
TAIR At2g32440; -.
Gene expression databases
ArrayExpress Q9C5Y2; -.
GermOnline AT2G32440; Arabidopsis thaliana.
Ontologies
GO
GO:0005789; Cellular component: endoplasmic reticulum membrane (inferred from electronic annotation from UniProtKB-SubCell).
QuickGo view.
Family and domain databases
InterPro IPR001128; Cyt_P450.
IPR002397; Cyt_P450_B.
Graphical view of domain structure.
Gene3D G3DSA:1.10.630.10; Cyt_P450; 1.
PANTHER PTHR19383; Cyt_P450; 1.
Pfam PF00067; p450; 1.
Pfam graphical view of domain structure.
PRINTS PR00359; BP450.
PR00385; P450.
PROSITE PS00086; CYTOCHROME_P450; 1.
BLOCKS Q9C5Y2.
Genome annotation databases
GeneID 817805; -.
GenomeReviews CT485783_GR; AT2G32440.
KEGG ath:AT2G32440; -.
NMPDR fig|3702.1.peg.10315; -.
Other
ProtoNet Q9C5Y2.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Endoplasmic reticulum; Heme; Iron; Membrane; Metal-binding; Monooxygenase; NADP; Oxidoreductase; Transmembrane.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   489  489     Ent-kaurenoic acid oxidase 2. PRO_0000052179
TRANSMEM   5    25  21     Potential. 
METAL   436   436        Iron (heme axial ligand) (By similarity). 
CONFLICT   288   288        E -> K (in Ref. 1; AAK11565). 
Sequence information
Length: 489 AA [This is the length of the unprocessed precursor] Molecular weight: 56750 Da [This is the MW of the unprocessed precursor] CRC64: 945F204F6CB251DA [This is a checksum on the sequence]
        10         20         30         40         50         60 
MTETGLILMW FPLIILGLFV LKWVLKRVNV WIYVSKLGEK KHYLPPGDLG WPVIGNMWSF 

        70         80         90        100        110        120 
LRAFKTSDPE SFIQSYITRY GRTGIYKAHM FGYPCVLVTT PETCRRVLTD DDAFHIGWPK 

       130        140        150        160        170        180 
STMKLIGRKS FVGISFEEHK RLRRLTSAPV NGPEALSVYI QFIEETVNTD LEKWSKMGEI 

       190        200        210        220        230        240 
EFLSHLRKLT FKVIMYIFLS SESEHVMDSL EREYTNLNYG VRAMGINLPG FAYHRALKAR 

       250        260        270        280        290        300 
KKLVAAFQSI VTNRRNQRKQ NISSNRKDML DNLIDVKDEN GRVLDDEEII DLLLMYLNAG 

       310        320        330        340        350        360 
HESSGHLTMW ATILMQEHPM ILQKAKEEQE RIVKKRAPGQ KLTLKETREM VYLSQVIDET 

       370        380        390        400        410        420 
LRVITFSLTA FREAKSDVQM DGYIIPKGWK VLTWFRNVHL DPEIYPDPKK FDPSRWEGYT 

       430        440        450        460        470        480 
PKAGTFLPFG LGSHLCPGND LAKLEISIFL HHFLLKYRVE RSNPGCPVMF LPHNRPKDNC 


LARITRTMP 

Q9C5Y2 in FASTA format

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