[1]	NUCLEOTIDE SEQUENCE [MRNA]. TISSUE=Pericarp; Lee B.-H., Choi D., Lee K.-W.; "Isolation and characterization of o-diphenol-O-methyltransferase cDNA clone in hot pepper (Capsicum annuum L.)."; J. Plant Biol. 41:9-14(1998).
[2]	NUCLEOTIDE SEQUENCE [MRNA]. STRAIN=cv. Chungyang; TISSUE=Root; Kim K.-W., Lee S.-W.; "Isolation and characterization of caffeic acid O-methyltrasferase cDNA from Capsicum annuum."; Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: Catalyzes the conversion of caffeic acid to ferulic acid and of 5-hydroxyferulic acid to sinapic acid. The resulting products may subsequently be converted to the corresponding alcohols that are incorporated into lignins.
CATALYTIC ACTIVITY: S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate = S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate.
PATHWAY: Aromatic compound metabolism; phenylpropanoid biosynthesis .
SUBUNIT: Homodimer (By similarity).
TISSUE SPECIFICITY: Fruit. Not expressed in leaf.
DEVELOPMENTAL STAGE: Expression increases during fruit development but decreases during ripening.
SIMILARITY: Belongs to the methyltransferase superfamily. Type 2 family. COMT subfamily.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

U83789; AAC17455.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF212316; AAG43822.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

T12259; T12259.

3D structure databases

HSSP

P28002; 1KYZ. [HSSP ENTRY / PDB]

SMR

Q9FQY8; 11-359.

ModBase

Q9FQY8.

Enzyme and pathway databases

BRENDA

2.1.1.68; 1261.

Ontologies

GO:0047763;	Molecular function: caffeate O-methyltransferase activity (inferred from electronic annotation from EC).
GO:0046983;	Molecular function: protein dimerization activity (inferred from electronic annotation from InterPro).
GO:0009809;	Biological process: lignin biosynthetic process (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR016461; O-MeTrfase_COMT_euk.
IPR001077; O_MeTrfase_2.
IPR012967; Plant_MeTrfase_dimerisation.
IPR011991; Wing_hlx_DNA_bd.
Graphical view of domain structure.

Gene3D

G3DSA:1.10.10.10; Wing_hlx_DNA_bd; 1.

Pfam

PF08100; Dimerisation; 1.
PF00891; Methyltransf_2; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF005739; O-mtase; 1.

Other

ProtoNet

Q9FQY8.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Lignin biosynthesis; Methyltransferase; S-adenosyl-L-methionine; Transferase.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 359`	`359`	`Caffeic acid 3-O-methyltransferase.`	`PRO_0000063196`
`REGION`	`126 132`	`7`	`Substrate binding (By similarity).`
`REGION`	`158 176`	`19`	`Substrate binding (By similarity).`
`ACT_SITE`	`265 265`		`Proton acceptor (By similarity).`
`BINDING`	`204 204`		`S-adenosyl-L-methionine; via carbonyl oxygen (By similarity).`
`BINDING`	`227 227`		`S-adenosyl-L-methionine (By similarity).`
`BINDING`	`247 247`		`S-adenosyl-L-methionine (By similarity).`
`BINDING`	`248 248`		`S-adenosyl-L-methionine; via amide nitrogen (By similarity).`
`BINDING`	`261 261`		`S-adenosyl-L-methionine (By similarity).`
`CONFLICT`	`71 71`		`V -> I (in Ref. 2; AAG43822).`
`CONFLICT`	`246 246`		`R -> G (in Ref. 2; AAG43822).`

Sequence information

Length: 359 AA [This is the length of the unprocessed precursor]

Molecular weight: 39433 Da [This is the MW of the unprocessed precursor]

CRC64: 70599AED75CE1730 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MDSTNQNLTQ TEDEAFLFAM QLASASVLPM VLKSALELDL LEIMAKAGPG AAISPSELAA 

        70         80         90        100        110        120 
QLPTKNPEAP VMLDRMLRLL ATYSVLNCTL RTLPDGRVER LYSLAPVCKL LTKNADGVSV 

       130        140        150        160        170        180 
APLLLMNQDK VLMESWYHLT DAVLDGGVPF NKAYGMTAFE YHGTDPRFNK VFNRGMSDHS 

       190        200        210        220        230        240 
TMTMKKILED YKGFEGLNSI VDVGGGTGAT VNMIVSKYPS IKGINFDLSH VIEDAPAYPG 

       250        260        270        280        290        300 
VEHVGRDMFV SVPKADAIFM KWICHDWSDE HCLKFLKNCY EALPANGKVL VAECILPETP 

       310        320        330        340        350 
DTSAATKNAV HVDIVMLAHN PGGKERTEKE FEALAKGAGF TGFRRACCAY QTWVMEFHK

Q9FQY8 in FASTA format

View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools