[1]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. STRAIN=cv. Columbia; DOI=10.1093/dnares/7.3.217; PubMed=10907853 [NCBI, ExPASy, EBI, Israel, Japan] Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.; "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."; DNA Res. 7:217-221(2000).
[2]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.; "Full-length cDNA from Arabidopsis thaliana."; Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
[3]	IDENTIFICATION, FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND INDUCTION. DOI=10.1038/sj.emboj.7600121; PubMed=15044947 [NCBI, ExPASy, EBI, Israel, Japan] Kushiro T., Okamoto M., Nakabayashi K., Yamagishi K., Kitamura S., Asami T., Hirai N., Koshiba T., Kamiya Y., Nambara E.; "The Arabidopsis cytochrome P450 CYP707A encodes ABA 8'-hydroxylases: key enzymes in ABA catabolism."; EMBO J. 23:1647-1656(2004).
[4]	FUNCTION, DEVELOPMENTAL STAGE, AND TISSUE SPECIFICITY. DOI=10.1104/pp.106.079475; PubMed=16543410 [NCBI, ExPASy, EBI, Israel, Japan] Okamoto M., Kuwahara A., Seo M., Kushiro T., Asami T., Hirai N., Kamiya Y., Koshiba T., Nambara E.; "CYP707A1 and CYP707A2, which encode abscisic acid 8'-hydroxylases, are indispensable for proper control of seed dormancy and germination in Arabidopsis."; Plant Physiol. 141:97-107(2006).

Comments

FUNCTION: Involved in the oxidative degradation of abscisic acid, but not in the isomerization of the produced 8'-hydroxyabscisic acid (8'-OH-ABA) to (-)-phaseic acid (PA).
CATALYTIC ACTIVITY: ⁺-abscisate + NADPH + O₂ = 8'-hydroxyabscisate + NADP⁺ + H₂O.
COFACTOR: Heme group (By similarity).
PATHWAY: Plant hormone degradation; abscisic acid degradation .
SUBCELLULAR LOCATION: Membrane; Single-pass membrane protein (Potential).
TISSUE SPECIFICITY: Mainly expressed in flowers. Lower expression in siliques, rosette leaves, roots and stems. Not expressed in dry seeds. Expressed in silique envelopes, but not in embryo or endosperm during the seed development.
DEVELOPMENTAL STAGE: Not induced after imbibition.
INDUCTION: By abscisic acid, dehydration and rehydration.
SIMILARITY: Belongs to the cytochrome P450 family.
SEQUENCE CAUTION:
- Sequence=BAB02968.1; Type=Erroneous gene model prediction;

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AP000419; BAB02968.1; ALT_SEQ; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AY085068; AAM61624.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_566628.1; -.

UniGene

At.38423

3D structure databases

ModBase

Q9LJK2.

Organism-specific databases

GeneFarm

1254; 94.

TAIR

At3g19270; -.

Family and domain databases

InterPro

IPR001128; Cyt_P450.
IPR002401; Cyt_P450_E_grp-I.
Graphical view of domain structure.

PANTHER

PTHR19383; Cyt_P450; 1.

PRINTS

PR00463; EP450I.
PR00385; P450.

PROSITE

PS00086; CYTOCHROME_P450; 1.

BLOCKS

Q9LJK2.

Genome annotation databases

GeneID

821461; -.

GenomeReviews

BA000014_GR; AT3G19270.

KEGG

ath:AT3G19270; -.

NMPDR

fig|3702.1.peg.14140; -.

Other

ProtoNet

Q9LJK2.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; Heme; Iron; Membrane; Metal-binding; Monooxygenase; NADP; Oxidoreductase; Stress response; Transmembrane.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 468`	`468`	`Abscisic acid 8'-hydroxylase 4.`	`PRO_0000288642`
`TRANSMEM`	`4 24`	`21`	`Potential.`
`METAL`	`415 415`		`Iron (heme axial ligand) (By similarity).`

Sequence information

Length: 468 AA [This is the length of the unprocessed precursor]

Molecular weight: 53922 Da [This is the MW of the unprocessed precursor]

CRC64: 0D9FA04292C062C7 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAEIWFLVVP ILILCLLLVR VIVSKKKKNS RGKLPPGSMG WPYLGETLQL YSQNPNVFFT 

        70         80         90        100        110        120 
SKQKRYGEIF KTRILGYPCV MLASPEAARF VLVTHAHMFK PTYPRSKEKL IGPSALFFHQ 

       130        140        150        160        170        180 
GDYHSHIRKL VQSSFYPETI RKLIPDIEHI ALSSLQSWAN MPIVSTYQEM KKFAFDVGIL 

       190        200        210        220        230        240 
AIFGHLESSY KEILKHNYNI VDKGYNSFPM SLPGTSYHKA LMARKQLKTI VSEIICERRE 

       250        260        270        280        290        300 
KRALQTDFLG HLLNFKNEKG RVLTQEQIAD NIIGVLFAAQ DTTASCLTWI LKYLHDDQKL 

       310        320        330        340        350        360 
LEAVKAEQKA IYEENSREKK PLTWRQTRNM PLTHKVIVES LRMASIISFT FREAVVDVEY 

       370        380        390        400        410        420 
KGYLIPKGWK VMPLFRNIHH NPKYFSNPEV FDPSRFEVNP KPNTFMPFGS GVHACPGNEL 

       430        440        450        460 
AKLQILIFLH HLVSNFRWEV KGGEKGIQYS PFPIPQNGLP ATFRRHSL

Q9LJK2 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools