[1]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. STRAIN=RK-1; PubMed=11040290 [NCBI, ExPASy, EBI, Israel, Japan] Gloeckner G., Rosenthal A., Valentin K.-U.; "The structure and gene repertoire of an ancient red algal plastid genome."; J. Mol. Evol. 51:382-390(2000).

Comments

FUNCTION: Catalyzes the formation of the isocyclic ring in chlorophyll biosynthesis. Mediates the cyclase reaction, which results in the formation of divinylprotochlorophyllide (Pchlide) characteristic of all chlorophylls from magnesium-protoporphyrin IX 13-monomethyl ester (MgPMME) (By similarity).
CATALYTIC ACTIVITY: Magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O₂ = 13¹-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP⁺ + H₂O.
CATALYTIC ACTIVITY: 13¹-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O₂ = 13¹-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADP⁺ + 2 H₂O.
CATALYTIC ACTIVITY: 13¹-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O₂ = divinylprotochlorophyllide + NADP⁺ + 2 H₂O.
COFACTOR: Iron (By similarity).
PATHWAY: Porphyrin metabolism; chlorophyll biosynthesis (light-independent).
SUBCELLULAR LOCATION: Plastid, chloroplast.
SIMILARITY: Belongs to the acsF family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AF022186; AAF12893.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_045201.1; -.

3D structure databases

ModBase

Q9TLR8.

Ontologies

GO:0005506;	Molecular function: iron ion binding (inferred from electronic annotation from HAMAP).
GO:0048529;	Molecular function: magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase activity (inferred from electronic annotation from HAMAP).
GO:0015979;	Biological process: photosynthesis (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_01840; -; 1.
PBIL [Tree]

InterPro

IPR008434; AcsF.
IPR003251; Rubrerythrin.
Graphical view of domain structure.

Pfam

PF02915; Rubrerythrin; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR02029; AcsF; 1.

BLOCKS

Q9TLR8.

Genome annotation databases

GeneID

800267; -.

Other

ProtoNet

Q9TLR8.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Chlorophyll biosynthesis; Chloroplast; Iron; Metal-binding; NADP; Oxidoreductase; Photosynthesis; Plastid.

Features

Feature table viewer

`Key`	`From To`	`Length`		`Description`	`FTId`
`CHAIN`	`1 354`	`354`		`Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase.`	`PRO_0000217541`

Sequence information

Length: 354 AA [This is the length of the unprocessed precursor]

Molecular weight: 42732 Da [This is the MW of the unprocessed precursor]

CRC64: E25EE64E12E045DA [This is a checksum on the sequence]

        10         20         30         40         50         60 
MVLNANHLQT KKTIKETLLA PRFYTTDFDE ISRYDISSNF EEIEAIVNEF RSDYNKKHFI 

        70         80         90        100        110        120 
RDEEFDRHWS QIDAQTKQMF VEFLERSCTA EFSGFLLYKE LARKLKEKMP VLAEGFLLMS 

       130        140        150        160        170        180 
RDEARHAGFL NKAMADFNLT LDLGFMTKNR RYTFFKPKFI FYATYLSEKI GYWRYITIYR 

       190        200        210        220        230        240 
HLEKHPEHRI YPIFKFFESW CQDENRHGDF FAAIVKSQPY LLEGFISKLW CRFFLLAVFA 

       250        260        270        280        290        300 
TMYLNDCQRG EFYKSIGLDP RQFDIYVIQK TNESAAKLFP TVLDLDNPRF FECLDKCACY 

       310        320        330        340        350 
NKLLLEIDDR EQPFFNQVLN VFYKFYLYFL LVLNFIMLYF LRTVNCNSFT RMVK

Q9TLR8 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools