NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=DSM 2278;
DOI=10.1111/j.1574-6968.1999.tb13399.x; PubMed=9933933 [NCBI, ExPASy, EBI, Israel, Japan]
Westenberg D.J., Braune A., Ruppert C., Mueller V., Herzberg C., Gottschalk G., Blaut M.;
"The F420H2-dehydrogenase from Methanolobus tindarius: cloning of the ffd operon and expression of the genes in Escherichia coli.";
FEMS Microbiol. Lett. 170:389-398(1999).

Comments

FUNCTION: Catalyzes the reversible reduction of methylene-H(4)MPT to methyl-H(4)MPT (By similarity).
CATALYTIC ACTIVITY: 5-methyltetrahydromethanopterin + coenzyme F420 = 5,10-methylenetetrahydromethanopterin + reduced coenzyme F420.
PATHWAY: One-carbon metabolism; methanogenesis from carbone dioxide; methyl-CoM from 5,10-methylene-H(4)MPT: step 1/2 .
SUBCELLULAR LOCATION: Cytoplasm (By similarity).
SIMILARITY: Belongs to the mer family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AJ011519; CAB56639.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

T45226; T45226.

3D structure databases

HSSP

Q50744; 1F07. [HSSP ENTRY / PDB]

SMR

Q9UXP0; 1-326.

ModBase

Q9UXP0.

Ontologies

GO:0005737;	Cellular component: cytoplasm (inferred from electronic annotation from HAMAP).
GO:0018537;	Molecular function: coenzyme F420-dependent N5,N10-methenyltetrahydromethanopterin reductase activity (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_01091; -; 1.
PBIL [Tree]

InterPro

IPR011251; Luciferase-like_bac.
IPR016048; Luciferase_mOase.
Graphical view of domain structure.

Gene3D

G3DSA:3.20.20.30; Luciferase_like; 1.

Pfam

PF00296; Bac_luciferase; 1.
Pfam graphical view of domain structure.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cytoplasm; Methanogenesis; One-carbon metabolism; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`		`Description`	`FTId`
`CHAIN`	`1 326`	`326`		`5,10-methylenetetrahydromethanopterin reductase.`	`PRO_0000084811`

Sequence information

Length: 326 AA [This is the length of the unprocessed precursor]

Molecular weight: 34043 Da [This is the MW of the unprocessed precursor]

CRC64: 16F3AB9733A45D82 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MTFGIEFVPS DPVLKIAHYA KLAEQQGFDN VWITDHYNNR DVYSTLTVLA MNTNSIKLGT 

        70         80         90        100        110        120 
GVTNPYTRNA AITASSIGSL NEISGGRAIL GLGPGEQATF DAMGISWEQP LTTTKESIAA 

       130        140        150        160        170        180 
IRGFLAGEKV TMDGDMIKFG GAKMAFKAGD VPIYMGAQGP KMLELAGEVS DGVLINASHP 

       190        200        210        220        230        240 
KDFEVAVKQI AAGAKKAGRD PKEVDVAAYA CFSIDKDAAK AKSAAQIVVA FIVAGSPDMV 

       250        260        270        280        290        300 
LERHGIDPAA KADIGGAIAK GDFGALMGGM VTDSMMDAFS ICGTPDDCKA RINELLDIGV 

       310        320 
TQIVAGSPIG PNKEKAIKLI GKEIIG

Q9UXP0 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools