[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Ha S.-H., Lee S.-W., Hwang Y.-S.; "Isolation and characterization of two differentially expressed hydroxy-methylglutaryl-CoA reductase genes from rice."; Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases.
[2]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. STRAIN=cv. Nipponbare; The international rice genome sequencing project (IRGSP) consortium; "Oryza sativa nipponbare chromosome 8 genomic DNA sequence."; Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases.
[3]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 95-561. STRAIN=cv. Nipponbare; DOI=10.1126/science.1081288; PubMed=12869764 [NCBI, ExPASy, EBI, Israel, Japan] The rice full-length cDNA consortium; "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."; Science 301:376-379(2003).

Comments

FUNCTION: Catalyzes the synthesis of mevalonate. The specific precursor of all isoprenoid compounds present in plants.
CATALYTIC ACTIVITY: (R)-mevalonate + CoA + 2 NADP⁺ = (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH.
PATHWAY: Metabolic intermediate biosynthesis; mevalonic acid biosynthesis; (R)-mevalonic acid from acetyl-CoA: step 3/3 .
PATHWAY: Context: Plant isoprenoid biosynthesis .
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein.
SIMILARITY: Belongs to the HMG-CoA reductase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AF110382; AAD38873.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AP004765; BAD10066.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AK060545; -; NOT_ANNOTATED_CDS; mRNA.	[EMBL / GenBank / DDBJ]

3D structure databases

HSSP

P04035; 1DQA. [HSSP ENTRY / PDB]

ModBase

Q9XHL5.

Organism-specific databases

Gramene

Q9XHL5; -.

Ontologies

GO:0005789;	Cellular component: endoplasmic reticulum membrane (inferred from electronic annotation from UniProtKB-SubCell).
QuickGo view.

Family and domain databases

InterPro

IPR002202; HMG_CoA_Rdtase_cat.
IPR004554; HMG_CoA_Rdtase_I_cat.
Graphical view of domain structure.

Gene3D

G3DSA:3.90.770.10; HMG-CoA_red; 1.

PANTHER

PTHR10572; HMG-CoA_red; 1.

Pfam

PF00368; HMG-CoA_red; 1.
Pfam graphical view of domain structure.

PRINTS

PR00071; HMGCOARDTASE.

TIGRFAMs

TIGR00533; HMG_CoA_R_NADP; 1.

PROSITE

PS00066; HMG_COA_REDUCTASE_1; 1.
PS00318; HMG_COA_REDUCTASE_2; 1.
PS01192; HMG_COA_REDUCTASE_3; 1.
PS50065; HMG_COA_REDUCTASE_4; 1.
PROSITE graphical view of domain structure (profiles).

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Endoplasmic reticulum; Glycoprotein; Isoprene biosynthesis; Membrane; NADP; Oxidoreductase; Transmembrane.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 561`	`561`	`3-hydroxy-3-methylglutaryl-coenzyme A reductase 3.`	`PRO_0000114447`
`TRANSMEM`	`25 45`	`21`	`Potential.`
`TRANSMEM`	`69 89`	`21`	`Potential.`
`REGION`	`90 145`	`56`	`Linker (By similarity).`
`REGION`	`146 561`	`416`	`Catalytic (By similarity).`
`ACT_SITE`	`240 240`		`Charge relay system (By similarity).`
`ACT_SITE`	`372 372`		`Charge relay system (By similarity).`
`ACT_SITE`	`448 448`		`Charge relay system (By similarity).`
`ACT_SITE`	`546 546`		`Proton donor (By similarity).`
`CARBOHYD`	`304 304`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`550 550`		`N-linked (GlcNAc...) (Potential).`
`CONFLICT`	`119 121`		`PPP -> RA (in Ref. 1; AAD38873).`
`CONFLICT`	`345 345`		`V -> E (in Ref. 1; AAD38873).`
`CONFLICT`	`538 538`		`A -> R (in Ref. 1; AAD38873).`

Sequence information

Length: 561 AA [This is the length of the unprocessed precursor]

Molecular weight: 59401 Da [This is the MW of the unprocessed precursor]

CRC64: B2A826D6A1BA0B1C [This is a checksum on the sequence]

        10         20         30         40         50         60 
MEVRRRAPLP PPPGRVQAGD ALPLPIRHTN LIFSALFAAS LAYLMRRWRE KIRSSTPLHV 

        70         80         90        100        110        120 
VGLAEMLAIF GLVASLIYLL SFFGIAFVQS IVSSSDDEEE DFLVGPARGS SAAAAVAPPP 

       130        140        150        160        170        180 
PPSSPAQCSL LGSPHDDAAR ERMPEEDEEI VSSVVAGKVP SYVLETKLGD CRRAAGIRRE 

       190        200        210        220        230        240 
AVRRITGRQI EGLPLDGFDY ASILGQCCEL PVGYVQLPVG IAGPLLLDGQ RFYVPMATTE 

       250        260        270        280        290        300 
GCLVASTNRG CKAIAESGGA VSVVLRDGMT RAPVARLPTA RRAAELKAFL EDSVNFNTLS 

       310        320        330        340        350        360 
MVFNRSSRFA RLQGVQCAMA GRNLYMRFSC CTGDAMGMNM VSKGVQNVLD YLQDDFPDMD 

       370        380        390        400        410        420 
VISISGNFCS DKKPAAVNWI EGRGKSVVCE AVIKEDVVKK VLKTNVQSLV ELNVIKNLAG 

       430        440        450        460        470        480 
SAVAGALGGF NAHASNIVTA IFIATGQDPA QNVESSHCIT MLEAVNDGRD LHISVTMPSI 

       490        500        510        520        530        540 
EVGTVGGGTQ LASQAACLDL LGVKGANRES PGSNARLLAT VVAGGVLAGE LSLLSALAAG 

       550        560 
QLVKSHMKYN RSSKDMSKVI S

Q9XHL5 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools