Search for
You are here: ExPASy CA  > Databases  > Around UniProtKB  > HAMAP

Home Proteomes Families Documents Downloads Links
HAMAP annotation rule: MF_01166

Send feedback new

General rule information

Accession MF_01166
Dates 11-NOV-2005 (Created)
25-NOV-2009 (Last updated, Version 19)
Data class Protein
Predictors HAMAP; MF_01166; [distribution of match scores in UniProtKB];[seed alignment for MF_01166]
Names ArnA


Propagated annotation

Identifier, protein and gene names
Identifier ARNA
Protein name
RecName: Full=Bifunctional polymyxin resistance protein arnA;
RecName: Full=UDP-4-amino-4-deoxy-L-arabinose formyltransferase;
EC=2.1.2.n1;
AltName: Full=UDP-L-Ara4N formyltransferase;
AltName: Full=ArnAFT;
RecName: Full=UDP-glucuronic acid oxidase, UDP-4-keto-hexauronic acid decarboxylating;
EC=1.1.1.n1;
AltName: Full=UDP-GlcUA decarboxylase;
AltName: Full=UDP-glucuronic acid dehydrogenase;
AltName: Full=ArnADH;
Gene name arnA
Comments
FUNCTION: Bifunctional enzyme that catalyzes the oxidative decarboxylation of UDP-glucuronic acid (UDP-GlcUA) to UDP-4-keto-arabinose (UDP-Ara4O) and the addition of a formyl group to UDP-4-amino-4-deoxy-L-arabinose (UDP-L-Ara4N) to form UDP-L-4-formamido-arabinose (UDP-L-Ara4FN). The modified arabinose is attached to lipid A and is required for resistance to polymyxin and cationic antimicrobial peptides (By similarity).
CATALYTIC ACTIVITY: UDP-alpha-D-glucuronate + NAD(+) = UDP-beta-L-threo-pentapyranos-4-ulose + CO(2) + NADH.
CATALYTIC ACTIVITY: 10-formyltetrahydrofolate + UDP-4-amino-4-deoxy-beta-L-arabinose = 5,6,7,8-tetrahydrofolate + UDP-4-deoxy-4-formamido-beta-L-arabinose.
PATHWAY: Nucleotide-sugar biosynthesis; UDP-4-deoxy-4-formamido-beta-L-arabinose biosynthesis; UDP-4-deoxy-4-formamido-beta-L-arabinose from UDP-alpha-D-glucuronate: step 1/3.
PATHWAY: Nucleotide-sugar biosynthesis; UDP-4-deoxy-4-formamido-beta-L-arabinose biosynthesis; UDP-4-deoxy-4-formamido-beta-L-arabinose from UDP-alpha-D-glucuronate: step 3/3.
PATHWAY: Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis.
SUBUNIT: Homohexamer, formed by a dimer of trimers (By similarity).
SIMILARITY: In the N-terminal section; belongs to the fmt family. UDP-L-Ara4N formyltransferase subfamily.
SIMILARITY: In the C-terminal section; belongs to the sugar epimerase family. UDP-glucuronic acid decarboxylase subfamily.
Cross-references
Pfam PF02911; Formyl_trans_C; 1;
PF00551; Formyl_trans_N; 1;
PF01370; Epimerase; 1;
Keywords
Antibiotic resistance, Lipid A biosynthesis, Lipid synthesis, Lipopolysaccharide biosynthesis, Methyltransferase, Multifunctional enzyme, NAD, Oxidoreductase, Transferase.
Gene Ontology
GO:0016616; Molecular function: oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor.
GO:0016742; Molecular function: hydroxymethyl-, formyl- and related transferase activity.
GO:0046493; Biological process: lipid A metabolic process.
Features
From: ARNA_ECOLI (P77398)
Key     From     To       Description   Condition   FTGroup
NP_BIND     368     369       NAD binding (By similarity)   D-[IV]  
REGION     Nter     304       Formyltransferase ArnAFT      
REGION (Optional)     86     88       10-formyltetrahydrofolate binding (By similarity)   H-x-I  
REGION     136     140       10-formyltetrahydrofolate binding (By similarity)   [VIT]-x(3)-D  
REGION     314     Cter       Dehydrogenase ArnADH      
REGION     432     433       UDP-glucuronate binding (By similarity)   T-S  
REGION     526     535       UDP-glucuronate binding (By similarity)   [KRQ]-x(6)-Q-x-R  
ACT_SITE     104     104       Proton donor; for formyltransferase activity (By similarity)   H  
ACT_SITE     434     434       Proton acceptor; for decarboxylase activity (By similarity)   E  
ACT_SITE     619     619       Proton donor; for decarboxylase activity (By similarity)   R  
BINDING     114     114       10-formyltetrahydrofolate (By similarity)   R  
BINDING     347     347       NAD (By similarity)   D  
BINDING     393     393       UDP-glucuronate; via carbonyl oxygen (By similarity)   A  
BINDING     398     398       UDP-glucuronate (By similarity)   Y  
BINDING     460     460       UDP-glucuronate (By similarity)   R  
BINDING     492     492       UDP-glucuronate (By similarity)   N  
BINDING     613     613       UDP-glucuronate (By similarity)   Y  
SITE     102     102       Transition state stabilizer (By similarity)   N  
SITE     140     140       Raises pKa of active site His (By similarity)   D  


Additional information

Size range: 654-687 amino acids
Related UniRules: MF_00182 (FMT)
Template: P77398 (ARNA_ECOLI); O52325 (ARNA_SALTY): [Recover all]
Scope: Bacteria; Gammaproteobacteria
Fusion: Nter: None; Cter: None
Duplicate: None
Plasmid encoded: None

View rule in raw text format (no links)



UniProtKB rule member sequences