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| HAMAP annotation rule: MF_01263 |
| Accession | MF_01263 |
| Dates | 29-JUL-2005 (Created) 25-NOV-2009 (Last updated, Version 7) |
| Data class | Protein |
| Predictors | HAMAP; MF_01263; [distribution of match scores in UniProtKB];[seed alignment for MF_01263] |
| Names | CCA_bact_type3 |
| Identifier | CCA |
| Protein name |
|
| Gene name | cca |
FUNCTION: Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate (By similarity).
CATALYTIC ACTIVITY: ATP + tRNA(n) = diphosphate + tRNA(n+1).
CATALYTIC ACTIVITY: CTP + tRNA(n) = diphosphate + tRNA(n+1).
COFACTOR: Magnesium (By similarity).
SUBUNIT: Homodimer (By similarity).
MISCELLANEOUS: A single active site specifically recognizes both ATP and CTP and is responsible for their addition (By similarity).
SIMILARITY: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily.
| Pfam |
PF01743; PolyA_pol; 1; |
ATP-binding,
Magnesium,
Metal-binding,
Nucleotide-binding,
Nucleotidyltransferase,
RNA repair,
RNA-binding,
Transferase,
tRNA processing.
GO:0000049; Molecular function: tRNA binding.
GO:0000166; Molecular function: nucleotide binding.
GO:0000287; Molecular function: magnesium ion binding.
GO:0004810; Molecular function: tRNA adenylyltransferase activity.
GO:0005524; Molecular function: ATP binding.
GO:0016437; Molecular function: tRNA cytidylyltransferase activity.
GO:0001680; Biological process: tRNA 3'-terminal CCA addition.
GO:0000166; Molecular function: nucleotide binding.
GO:0000287; Molecular function: magnesium ion binding.
GO:0004810; Molecular function: tRNA adenylyltransferase activity.
GO:0005524; Molecular function: ATP binding.
GO:0016437; Molecular function: tRNA cytidylyltransferase activity.
GO:0001680; Biological process: tRNA 3'-terminal CCA addition.
| From: CCA_BACST (Q7SIB1) | ||||||||||
| Key | From | To | Description | Condition | FTGroup | |||||
| METAL | 40 | 40 | Magnesium (By similarity) | D | ||||||
| METAL | 42 | 42 | Magnesium (By similarity) | D | ||||||
| BINDING | 27 | 27 | ATP or CTP; via amide nitrogen (By similarity) | G | ||||||
| BINDING | 30 | 30 | ATP or CTP (By similarity) | R | ||||||
| BINDING | 111 | 111 | ATP or CTP (By similarity) | R | ||||||
| BINDING | 154 | 154 | ATP or CTP (By similarity) | D | ||||||
| BINDING | 157 | 157 | ATP or CTP (By similarity) | R | ||||||
| BINDING | 160 | 160 | ATP or CTP (By similarity) | R | ||||||
| BINDING | 163 | 163 | ATP or CTP (By similarity) | R | ||||||
| Size range: | 375-407 amino acids |
| Related UniRules: | None |
| Template: | Q7SIB1 (CCA_BACST); P42977 (CCA_BACSU): [Recover all] |
| Scope: | Bacteria; Firmicutes |
| Fusion: | Nter: None; Cter: None |
| Duplicate: | None |
| Plasmid encoded: | None |
| Comments: | The CCA-adding enzyme family consists of 4 different subfamilies: 3 bacterial subfamilies (MF_01261, MF_01262 and MF_01263) and 1 archaeal subfamily (MF_01264). |
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