ExPASy Proteomics Server

Accession	MF_01268
Dates	2-MAY-2006 (Created) 25-NOV-2009 (Last updated, Version 8)

Data class

Protein

Predictors

HAMAP; MF_01268; [distribution of match scores in UniProtKB];[seed alignment for MF_01268]

Names

Fae_Hps

Identifier

FAEHP

Protein name

RecName: Full=Bifunctional enzyme fae/hps; RecName: Full=Formaldehyde-activating enzyme; Short=Fae; EC=4.3.-.-; RecName: Full=3-hexulose-6-phosphate synthase; Short=HPS; EC=4.1.2.43; AltName: Full=D-arabino-3-hexulose-6-phosphate formaldehyde lyase;

Gene name

fae-hps

FUNCTION: Catalyzes the condensation of formaldehyde with tetrahydromethanopterin (H(4)MPT) to 5,10-methylenetetrahydromethanopterin (By similarity).

FUNCTION: Catalyzes the formation of ribulose-5-phosphate and formaldehyde from 3-hexulose-6-phosphate (By similarity).

CATALYTIC ACTIVITY: D-arabino-hex-3-ulose 6-phosphate = D-ribulose 5-phosphate + formaldehyde.

PATHWAY: Carbohydrate biosynthesis; D-ribose 5-phosphate biosynthesis.

SIMILARITY: In the N-terminal section; belongs to the formaldehyde-activating enzyme family.

SIMILARITY: In the C-terminal section; belongs to the HPS/KGPDC family. HPS subfamily.

Pfam	PF00215; OMPdecase; 1;

Carbohydrate metabolism, Lyase, Multifunctional enzyme.

GO:0016832; Molecular function: aldehyde-lyase activity.
GO:0016840; Molecular function: carbon-nitrogen lyase activity.
GO:0016051; Biological process: carbohydrate biosynthetic process.

From: FAEHP_METBF (Q46DY5)
Key		From		To		Description		Condition		FTGroup
REGION		Nter		161		Formaldehyde-activating enzyme
REGION		162		Cter		3-hexulose-6-phosphate synthase
ACT_SITE		17		17		Proton donor (By similarity)		H
BINDING		19		19		Substrate (By similarity)		D
BINDING		48		48		Substrate; via carbonyl oxygen (By similarity)		L
BINDING		66		66		Substrate (By similarity)		K
BINDING		68		68		Substrate (By similarity)		T
BINDING		83		83		Substrate (By similarity)		Q

Size range:	381-406 amino acids
Related UniRules:	None
Template:	Q46DY5 (FAEHP_METBF); Q58842 (FAEHP_METJA): [Recover all]
Scope:	Archaea
Fusion:	Nter: None; Cter: None
Duplicate:	None
Plasmid encoded:	None
Comments:	In METJA, lacks the active site His and substrate binding sites expected to be involved in fae activity. Monofunctional fae enzyme exists in methylotrophic bacteria, where it is involved in energy metabolism and formaldehyde detoxification. Monofunctional HPS enzyme exists in bacteria, but functions in the opposite direction (condensation of ribulose 5-phosphate with formaldehyde), and is involved in C1 assimilation and/or formaldehyde detoxification. METBF possesses the bifunctional fae/hps enzyme, and also the two monofunctional enzymes fae and hps. FT lines are propagated from Methylobacterium extorquens fae enzyme, whose X-ray structure is known.