ExPASy Proteomics Server

Accession	MF_02016
Dates	15-OCT-2008 (Created) 25-NOV-2009 (Last updated, Version 6)

Data class

Protein

Predictors

HAMAP; MF_02016; [distribution of match scores in UniProtKB];[seed alignment for MF_02016]

Names

MltF

Identifier

MLTF

Protein name

RecName: Full=Membrane-bound lytic murein transglycosylase F; EC=4.2.2.n1; AltName: Full=Murein lyase F; Flags: Precursor;

Gene name

mltF

FUNCTION: Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the peptidoglycan (PG) sacculus. Their lytic action creates space within the PG sacculus to allow for its expansion as well as for the insertion of various structures such as secretion systems and flagella (By similarity).

CATALYTIC ACTIVITY: Exolytic cleavage of the (1->4)-beta-glycosidic linkage between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) residues in peptidoglycan, from either the reducing or the non-reducing ends of the peptidoglycan chains, with concomitant formation of a 1,6-anhydrobond in the MurNAc residue.

SUBCELLULAR LOCATION: Cell outer membrane; Peripheral membrane protein. Note=Attached to the inner leaflet of the outer membrane (By similarity).

DOMAIN: The N-terminal domain does not have lytic activity and probably modulates enzymatic activity. The C-terminal domain is the catalytic active domain (By similarity).

SIMILARITY: In the N-terminal section; belongs to the bacterial solute-binding protein 3 family.

SIMILARITY: In the C-terminal section; belongs to the transglycosylase slt family.

PROSITE	PS00922; TRANSGLYCOSYLASE; 1;
Pfam	PF00497; SBP_bac_3; 1; PF01464; SLT; 1;
General	Signal; -; 1; trigger=Yes;

Cell membrane, Cell outer membrane, Cell wall biogenesis/degradation, Lyase, Membrane, Signal.

GO:0016998; Biological process: cell wall macromolecule catabolic process.
GO:0008933; Molecular function: lytic transglycosylase activity.
GO:0009279; Cellular component: cell outer membrane.

From: MLTF_ECOLI (P0AGC5)
Key		From		To		Description		Condition		FTGroup
REGION		22		269		Non-LT domain (By similarity)
REGION		270		Cter		LT domain (By similarity)
ACT_SITE		314		314		By similarity		E

Size range:	450-537 amino acids
Related UniRules:	None
Template:	P0AGC5 (MLTF_ECOLI)
Scope:	Bacteria; Proteobacteria
Fusion:	Nter: None; Cter: None
Duplicate:	in ALHEH
Plasmid encoded:	None